SeQuant® is moving.

The application collection, scientific publication library, and
HILIC retention prediction tool are expanding and moving to the Merck Millipore website.
You´re most welcome to download instructions and brochures for products like ZIC®-HILIC,
ZIC®-cHILIC and ZIC®-pHILIC columns, plus SAMS™ & CARS™ suppressor system at our new home.



For HPLC Separation of Polar Compounds

The ZIC®-HILIC column is the ideal choice for separation of polar and hydrophilic compounds as it is truly orthogonal to reversed phase chromatography (RPLC) columns. Analytes such as amino acids, peptides, carbohydrates, plant extracts and various other polar compounds that usually have little or no retention and may be affected by wettability problems in RPLC, generally have strong retention on the ZIC®-HILIC column.

The zwitterionic ZIC®-HILIC stationary phase is attached to porous silica and is available in columns from capillary to preparative dimensions. The separation is achieved by a hydrophilic partitioning mechanism superimposed on weak electrostatic interactions. Typical eluents contain a high content of an organic solvent (e.g., acetonitrile) mixed with buffer, i.e., comparable to RPLC eluents, however, water being the strong solvent. Also, the low content of water in the eluents enables higher detection sensitivity in many LC/MS applications.

NEW! Complementary Selectivity - SeQuant® ZIC®-cHILIC


Product brochure for ZIC®-HILIC and ZIC®-pHILIC

Recent applications with ZIC®-HILIC

ZIC-HILIC Separation of Carnitine and impurity (114 kB)
ZIC-HILIC Separation of Metformin Melamine and Cyanoguanidine (101 kB)
ZIC-HILIC Separation of Temozolomide and related impurities (112 kB)

Many more ZIC®-HILIC applications are available here.

Support documents for ZIC®-HILIC

For all who expect more - SeQuant ZIC-HILIC HPLC Columns (1188 kB)
ZIC-HILIC Analytical HPLC Columns (94 kB)
ZIC-HILIC Capillary HPLC Columns (83 kB)
ZIC-HILIC Semi-preparative HPLC Columns (82 kB)
A Practical Guide to HILIC (1534 kB)

More downloads for ZIC®-HILIC are available here.




Contact your local distributor for prices and ordering.



Common questions
about ZIC®-HILIC

Browse our FAQ or
e-mail the SeQuant Support Team.

Technology Tutorial

What is HILIC?

Learn online or
request the booklet
A Practical Guide


Separation of
polar compounds
using ZIC®-HILIC

Browse the list of applications.